human single-chain variable fragment ab (scfv (AxioMx Inc)
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Human Single Chain Variable Fragment Ab (Scfv, supplied by AxioMx Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Use of Epivolve phage display to generate a monoclonal antibody with opsonic activity directed against a subdominant epitope on extracellular loop 4 of Treponema pallidum BamA (TP0326)
Journal: Frontiers in Immunology
doi: 10.3389/fimmu.2023.1222267
Figure Legend Snippet: Schematic of mAb generation using Epivolve. A peptide incorporating a non-native amino acid at a desired site is used to pan a scFv phage library for peptide binders. Phages that bind the modified, but not the native, peptide undergo AXM mutagenesis to generate phages that recognize the native peptide +/− the modified peptide with high affinity.
Techniques Used: Modification, Mutagenesis
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Plasmid Preparation:Article Title: Use of Epivolve phage display to generate a monoclonal antibody with opsonic activity directed against a subdominant epitope on extracellular loop 4 of Treponema pallidum BamA (TP0326) Article Snippet: The template plasmid for all phage display libraries is a derivative of the phagemid pIT2 ( ) with a human single-chain variable fragment Ab (scFv) fused to the coat protein III of Article Title: Use of Epivolve phage display to generate a monoclonal antibody with opsonic activity directed against a subdominant epitope on extracellular loop 4 of Treponema pallidum BamA (TP0326) Article Snippet: The template plasmid for all phage display libraries is a derivative of the phagemid pIT2 ( ) with a human single-chain variable fragment Ab (scFv) fused to the coat protein III of Construct:Article Title: Use of Epivolve phage display to generate a monoclonal antibody with opsonic activity directed against a subdominant epitope on extracellular loop 4 of Treponema pallidum BamA (TP0326) Article Snippet: The template plasmid for all phage display libraries is a derivative of the phagemid pIT2 ( ) with a human single-chain variable fragment Ab (scFv) fused to the coat protein III of Article Title: Use of Epivolve phage display to generate a monoclonal antibody with opsonic activity directed against a subdominant epitope on extracellular loop 4 of Treponema pallidum BamA (TP0326) Article Snippet: The template plasmid for all phage display libraries is a derivative of the phagemid pIT2 ( ) with a human single-chain variable fragment Ab (scFv) fused to the coat protein III of Modification:Article Title: Use of Epivolve phage display to generate a monoclonal antibody with opsonic activity directed against a subdominant epitope on extracellular loop 4 of Treponema pallidum BamA (TP0326) Article Snippet: The template plasmid for all phage display libraries is a derivative of the phagemid pIT2 ( ) with a human single-chain variable fragment Ab (scFv) fused to the coat protein III of Article Title: Use of Epivolve phage display to generate a monoclonal antibody with opsonic activity directed against a subdominant epitope on extracellular loop 4 of Treponema pallidum BamA (TP0326) Article Snippet: The template plasmid for all phage display libraries is a derivative of the phagemid pIT2 ( ) with a human single-chain variable fragment Ab (scFv) fused to the coat protein III of Mutagenesis:Article Title: Use of Epivolve phage display to generate a monoclonal antibody with opsonic activity directed against a subdominant epitope on extracellular loop 4 of Treponema pallidum BamA (TP0326) Article Snippet: The template plasmid for all phage display libraries is a derivative of the phagemid pIT2 ( ) with a human single-chain variable fragment Ab (scFv) fused to the coat protein III of Article Title: Use of Epivolve phage display to generate a monoclonal antibody with opsonic activity directed against a subdominant epitope on extracellular loop 4 of Treponema pallidum BamA (TP0326) Article Snippet: The template plasmid for all phage display libraries is a derivative of the phagemid pIT2 ( ) with a human single-chain variable fragment Ab (scFv) fused to the coat protein III of |